Mumia quercus sp. nov., isolated from the root of Quercus variabilis. Issue 4 (12th April 2023)

• Record Type: Journal Article
• Authors: Chen, Hongmin; Cao, Tingting; Wang, Teng; Gao, Congting; Yang, Jingquan; Dong, Qingyan; Shen, Yue; Liu, Chongxi
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-12
• Subjects: Mumia quercus sp. nov. -- polyphasic taxonomy -- Quercus variabilis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005776
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel endophytic actinomycete, designated strain NEAU-365 T, was isolated from the root of Quercus variabilis collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365 T belonged to the genus Mumia but clearly differs from the currently recognized species Mumia zhuanghuii Z350 T (99.31 %), Mumia xiangluensis NEAU-KD1 T (98.82 %) and Mumia flava MUSC 201 T (97.78 %). Phylogenetic tree analysis revealed that strain NEAU-365 T clustered with the type strains of the genus Mumia . The genome size was 4.1 Mbp with a DNA G+C content of 71.2 mol%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain NEAU-365 T and those of M. zhuanghuii Z350 T (27.5 and 84.0 %), M. xiangluensis NEAU-KD1 T (23.4 and 80.9 %) and M. flava MUSC 201 T (20.9 and 77.7 %) were below the recommended thresholds for species delineation. Cells were observed to be irregular cocci shaped. The cell wall contained ll -diaminopimelic acid and the whole-cell sugars were galactose and rhamnose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unidentified phospholipids. The predominant menaquinone was detected as MK-9(H4 ). The major fatty acids were C16 : 0, C18 : 0, C18 : 1 ω9 c and 10-methyl C18 : 0 . On the basis of genotypic and phenotypic differences from members of the genus Mumia, a novel species, Mumia quercus sp. nov. is proposed. The type strain is NEAU-365 T (=CCTCC AA 2021033 T =JCM 35005 T ).