Sphaerisporangium perillae sp. nov., isolated from the root of Perilla frutescens (Linn.) Britt. Issue 4 (12th April 2023)

• Record Type: Journal Article
• Authors: Wang, Teng; Gao, Congting; Chen, Hongmin; Yang, Jingquan; Du, Rui; Zuo, Gengxin; Zhao, Junwei; Xiang, Wensheng; Liu, Chongxi
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-12
• Subjects: Sphaerisporangium perillae sp. nov. -- Perilla frutescens (Linn.) Britt -- protease; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005773
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel protease-producing actinomycete, designated strain NEAU-ZS1 T, was isolated from the root of Perilla frutescens (Linn.) Britt collected from Jiamusi, Heilongjiang, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-ZS1 T belonged to the genus Sphaerisporangium and was most closely related to ' Sphaerisporangium corydalis ' NEAU-YHS15 T (99.2%) and Sphaerisporangium cinnabarinum JCM 3291 T (99.0%). Phylogenetic tree analysis revealed that strain NEAU-ZS1 T formed a monophyletic clade with ' S. corydalis ' NEAU-YHS15 T . The genome size was 9.3 Mbp with a DNA G+C content of 70.3 mol%. Digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values between the genome sequence of strain NEAU-ZS1 T and those of ' S. corydalis ' NEAU-YHS15 T (28.6, 83.9 and 79.1 %) and S. cinnabarinum JCM 3291 T (18.5, 70.6 and 50.2 %) were below the recommended thresholds for species delineation. The strain formed spherical spore vesicles produced on the aerial hyphae. The cell wall contained meso -diaminopimelic acid and the whole-cell sugars were glucose and madurose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified glycolipid. The menaquinones were MK-9(H4 ), MK-9(H6 ) and MK-9(H2 ). The major fatty acids were iso-C16 : 0, C16 : 1 ω5 c, 10-methy C17 : 0 and C17 : 1 ω7 c . On the basis of the results of a polyphasic taxonomic study, it is concluded that strain NEAU-ZS1 T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium perillae sp. nov. is proposed. The type strain is NEAU-ZS1 T (=CCTCC AA 2021019 T = JCM 35655 T ).