Synergetic effect of lytic polysaccharide monooxygenase from Thermobifida fusca on saccharification of agrowastes. (June 2023)

• Record Type: Journal Article
• Authors: Park, Hyun June; Gwon, Seung Yeon; Lee, Jeongmi; Koo, Na Kyeong; Min, Kyoungseon
• Is Part Of: Bioresource technology. Volume 378(2023)
• Publication Date: 2023-06
• Subjects: LPMO from Thermobifida fusca -- Recombinant expression -- Synergistic effect with cellulase -- Agricultural waste; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2023.129015
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: TfLPMO functioned on various cellulosic and hemicellulosic substrates. TfLPMO with cellulase showed a synergistic effect on saccharification of agrowaste. TfLPMO boosted rice straw-derived reducing sugars production by 19.2% TfLPMO boosted corncob-derived reducing sugars by 14.1% Abstract: Saccharification is one of the most noteworthy processes in biomass-based biorefineries. In particular, the lytic polysaccharide monooxygenase has recently emerged as an oxidative cleavage-recalcitrant polysaccharide; however, there is insufficient information regarding its application to actual biomass. Accordingly, this study focused optimizing the recombinant expression level of a bacterial lytic polysaccharide monooxygenase from Thermobifida fusca (TfLPMO), which was characterized as a cellulolytic enzyme. Finally, the synergistic effect of the lytic polysaccharide monooxygenase and a commercial cellulase cocktail on the saccharification of agrowaste was investigated. TfLPMO functioned on various cellulosic and hemicellulosic substrates, and the combination of TfLPMO with cellulase exhibited a synergistic effect on the saccharification of agrowastes, resulting in a 19.2% and 14.1% increase in reducing sugars from rice straw and corncob, respectively. The results discussed herein can lead to an in-depth understanding of enzymatic saccharification and suggest viable options for valorizing agrowastes as renewable feedstocks in biorefineries.