Detection of pathological response of axillary lymph node metastasis after neoadjuvant chemotherapy in breast cancer using multiphoton microscopy. Issue 4 (20th December 2022)

• Record Type: Journal Article
• Authors: Han, Zhonghua; Huang, Xingxin; Kang, Deyong; Fu, Fangmeng; Zhang, Shichao; Zhan, Zhenlin; Chen, Jianxin; Li, Lianhuang; Wang, Chuan
• Is Part Of: Journal of biophotonics. Volume 16:Issue 4(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-20
• Subjects: axillary lymph node -- breast cancer -- multiphoton microscopy -- neoadjuvant chemotherapy; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202200274
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Neoadjuvant treatment is often considered in breast cancer patients with axillary lymph node involvement, but most of patients do not have a pathologic complete response to therapy. The detection of residual nodal disease has a significant impact on adjuvant therapy recommendations which may improve survival. Here, we investigate whether multiphoton microscopy (MPM) could identify the pathological changes of axillary lymphatic metastasis after neoadjuvant chemotherapy in breast cancer. And furthermore, we find that there are obvious differences in seven collagen morphological features between normal node and residual axillary disease by combining with a semi‐automatic image processing method, and also find that there are significant differences in four collagen features between the effective and no‐response treatment groups. These research results indicate that MPM may help estimate axillary treatment response in the neoadjuvant setting and thereby tailor more appropriate and personalized adjuvant treatments for breast cancer patients. Abstract : Here, we try to use multiphoton microscopy (MPM) to label‐free assess the pathological changes of axillary lymph node metastasis induced by neoadjuvant chemotherapy, and also perform image analysis on multiphoton images to provide quantitative information to aid in monitoring the collagen changes between normal and abnormal lymph nodes as well as the diseased nodes with and without treatment response. We hope MPM will become an auxiliary diagnostic tool to help clinicians to rapidly conduct histopathologic examination without staining dye.