Acute and sub-acute toxicity evaluation of Merremia tridentata (L.) stem extract on mice. (May 2023) - Canh Pham, Em
Abstract: The acute and sub-acute toxicity studies were performed in male and female Swiss albino mice as per the guidelines mentioned in OECD. The oral administration of M. tridentata stem extract (MSE) showed no treatment-related mortality and body weight change in mice up to the single dose of 30, 000 mg/kg body weight in the acute toxicity study and up to a dose of 30, 000 mg/kg/day body weight in the sub-acute toxicity study. Moreover, the clinical signs, body weight, gross pathology, organ weight, hematology (except for platelet count), biochemical analysis, and histopathology did not show significant variation at a medium dose of 15, 000 mg/kg/day body weight compared to the control group. However, toxicological signs in behavior, very mild interstitial nephritis, as well as significant variation in platelet count and total protein parameters were observed at a dose of 30, 000 mg/kg/day in the 28-day oral toxicity study. Thus, the no-observed-adverse-effect level was determined at a dose of 15, 000 mg/kg/day body weight. Based on study results, it is concluded that MSE showed LD50 of greater than 5000 mg/kg/day body weight. Hence, this could be a potential candidature as a future safe pharmaceutical product. Graphical abstract: Image 1 Highlights: Provides valuable data on the acute and sub-acute toxicity of MSE in mice. MSE is a safe drug up to a dose of 30, 000 mg/kg body weight in acute toxicity study. NOAEL was determined at a dose of 15, 000 mg/kg/day body weight. MSE is safe to use at a dose of 15, 000 mg/kg/day for a period of 28 days.