In vitro evaluation of ginsenoside Rg1 immunostimulating effect in bovine mononuclear cells. (May 2023)

• Record Type: Journal Article
• Authors: Silvestrini, Paula; Beccaria, Camila; Renna, María S.; Engler, Carolina; Simonutti, Valeria; Cellone, Ivana; Calvinho, Luis F.; Dallard, Bibiana E.; Baravalle, Celina
• Is Part Of: Research in veterinary science. Volume 158(2023)
• Page Start: 1
• Page End: 12
• Publication Date: 2023-05
• Subjects: Ginsenoside Rg1 -- Immunostimulating effect -- Bovine mammary secretion mononuclear cells -- Bovine peripheral blood mononuclear cells; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2023.03.003
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: The aim of this study was to evaluate the immunomodulatory effect of ginsenoside Rg1 on mammary secretion and peripheral blood mononuclear cells (MSMC and PBMC, respectively). The mRNA expression of TLR2, TLR4 and selected cytokines were evaluated on MSMC after Rg1 treatment. Also, TLR2 and TLR4 protein expression was evaluated on MSMC and PBMC after Rg1 treatment. Phagocytic activity and capacity, ROS production and MHC-II expression were evaluated on MSMC and PBMC after Rg1 treatment and co-culture with Staphylococcus aureus strain 5011. Rg1 induced mRNA expression of TLR2, TLR4, TNF-α, IL-1β, IL-6 and IL-8 in groups treated with different concentrations and at different times in MSMC, and induced TLR2 and TLR4 protein expression in MSMC and PBMC. Rg1 increased phagocytic capacity and ROS production in MSMC and PBMC. Rg1 increased MHC-II expression by PBMC. However, Rg1 pre-treatment had no effect on cells co-cultured with S. aureus . In conclusion, Rg1 was able to stimulate several sensing and effector activities in these immune cells. Highlights: Ginsenoside Rg1 induced TLR2, TLR-4 and cytokines mRNA expression in MSMC. Rg1 induced TLR2 and TLR4 protein expression in MSMC and PBMC. Rg1 enhanced the phagocytic capacity in PBMC and ROS production in MSMC and PBMC. Rg1 increased MHC-II expression on PBMC but not in cells cultured with S. aureus. Rg1 can exert stimulating effects in bovine cells involved in innate immune responses.