Intracellular Formation of Hemicyanine Nanoparticle Enhances Tumor‐Targeting Photoacoustic Imaging and Photothermal Therapy. Issue 9 (15th January 2023)

• Record Type: Journal Article
• Authors: Zhang, Lele; Wang, Minghui; Wu, Fangzheng; Liu, Li; Ren, Xingxing; Hai, Zijuan
• Is Part Of: Advanced healthcare materials. Volume 12:Issue 9(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-15
• Subjects: hemicyanine dyes -- photoacoustic imaging -- photothermal therapy -- self‐assembly -- tumor‐targeting; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202202676
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Alkaline phosphatase (ALP) is a tumor marker for early diagnosis and treatment. Tumor targeting can recognize and fight tumor cells more accurately from healthy cells. Glycyrrhetinic acid (GA) is a targeting ligand of liver tumors. Photoacoustic imaging (PAI) and photothermal therapy (PTT) are promising techniques for tumor diagnosis and treatment. The outstanding characteristics of Hemicyanine (HCy) dye make it suitable for tumor diagnosis and treatment. However, using HCy nanoparticle (HCy NP) for liver tumor‐targeting PAI and PTT has not been reported. Herein, Probe‐1 is developed to enhance PAI and PTT of liver tumors due to GA targeting and intracellular ALP‐instructed self‐assembly of HCy NP. Compared to Probe‐2 without self‐assembly ability, Probe‐1 displays a 4.6‐fold higher PAI signal or 1.7‐fold lower half inhibitory concentrations in HepG2 cells. Moreover, Probe‐1 shows extended retention time (10 vs 6 h) and 2.1‐fold higher PAI signal than Probe‐2 in HepG2 tumors. The HepG2 tumors in Group Probe‐1 obviously increase 18 °C ( T max : 55 °C) with a 3.3‐fold decreased volume while that in Group Probe‐2 mildly increase 9.8 °C ( T max : 46.8 °C) with a 4.3‐fold increased volume. It is envisioned that this smart self‐assembly strategy can be easily adjusted for PAI and PTT of more tumors. Abstract : H2 PO3 ‐HCy‐FFEGA enter into tumor cells by binding with glycyrrhetinic acid （GA） receptor on the cell membrane. The overexpressed alkaline phosphatase (ALP) catalyzes the dephosphorylation to release OH‐HCy‐FFEGA with photoacoustic imaging (PAI) and photothermal therapy (PTT). OH‐HCy‐FFEGA self‐assembles into hemicyanine nanoparticle (HCy NP) by π – π stacking, which further enhances PAI and PTT.