ARMOR: Auto‐Assembled Resilient Biomimetic Calcified Ornaments for Selective Cell Protection by Dual‐Aptamer‐Driven Hybridization Chain Reaction. (3rd March 2023) - Yu, Xiyuan
Abstract: Unlike plant and microbial cells having cell walls, the outermost layer of mammalian cell is a delicate, two‐layered structure of phospholipids with proteins embedded, which is susceptible to environmental changes. It is necessary to create an "armor" on cell surface to protect cell integrity. Here, we propose an A uto‐assembled R esilient bioM imetic calcified OR naments (ARMOR) strategy driven by dual‐aptamer‐based hybridization chain reaction (HCR) and Ca 2+ assisted calcification for selective cell protection. This co‐recognition design enhances the selectivity and leverages robust in situ signal amplification by HCR to improve the sensitivity. The calcified shell is cogenerated by crosslinking the alginate‐HCR product with Ca 2+ ion. ARMOR has high efficiency for shielding cells from environmental assaults, which can be applied to circulating tumor cell (CTC) protection, isolation, and identification, maintaining the native state and intact genetic information for downstream analysis. Abstract : The Auto‐assembled Resilient bioMimetic calcified ORnaments (ARMOR) strategy was created by dual‐aptamer‐based HCR and Ca 2+ assisted calcification for selective cell protection. The ARMOR can shield cells from external assaults and enhance the survival rate of the protected cells in an unfavorable environment. The ARMOR‐based cell capture strategy can obtain more intact genetic information and provide a new method for clinical CTC analysis.