An electrochemical biosensor to identify the phenotype of aggressive breast cancer cells. Issue 26 (14th March 2023)

• Record Type: Journal Article
• Authors: Wang, Lin; Xie, Haojie; Zhou, Xinyi; Lin, Yuxin; Qin, Yujia; Yang, Jie; Zhao, Jing; Li, Genxi
• Is Part Of: Chemical communications. Volume 59:Issue 26(2023)
• Page Start: 3890
• Page End: 3893
• Publication Date: 2023-03-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d3cc00611e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : An electrochemical biosensor is fabricated to identify the phenotype of aggressive breast cancer cells via detection of HER-2 together with MMP-2. Abstract : Identifying the phenotype of aggressive breast cancer (BC) cells is vital for the effectiveness of surgical intervention and standard-of-care therapy. HER-2 is overexpressed in aggressive BC and MMP-2 is a crucial indicator of invasiveness and metastasis of BC, so we have proposed an electrochemical biosensor in this work to identify the phenotype of aggressive BC cells via detection of HER-2 together with MMP-2 by designing a dual-trapping peptide and a metal organic framework (MOF)-based probe. Specifically, the designed peptide contains both a HER-2 recognition sequence and MMP-2-specific substrate, while the MOF-based probe (AuNPs@HRP@ZIF-8), prepared by loading horseradish peroxidase (HRP) and gold nanoparticles (AuNPs) on ZIF-8, can also combine with the peptide. Consequently, sensitive and specific detection of both HER-2 and MMP-2 can be achieved in the wide range from 50 fg mL −1 to 50 ng mL −1 and 10 fg mL −1 to 10 ng mL −1, respectively, and the biosensor can distinguish HER-2 + BC cells and evaluate the invasion capability, which might be extended to provide a method for the accurate identification of tumor features in BC subtypes.