A hairpin probe-mediated exponential amplification reaction for highly sensitive and specific detection of microRNAs. Issue 28 (7th March 2023)

• Record Type: Journal Article
• Authors: Yu, Hongyan; Weng, Zhi; Zhou, Xi; Bai, Dan; Luo, Wang; Han, Xiaole; Song, Lin; Liu, Qian; Li, Junjie; Yang, Yujun; Guo, Yongcan; Lv, Ke; Xie, Guoming
• Is Part Of: Chemical communications. Volume 59:Issue 28(2023)
• Page Start: 4158
• Page End: 4161
• Publication Date: 2023-03-07
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d3cc00241a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : In this work, we propose hairpin probe-mediated exponential amplification reaction (HEAR) strategy that combines DNA strand displacement with "who triggers, who gets generated" mode, providing single-base discrimination and reduced background signal. Abstract : In this work, we propose a hairpin probe-mediated exponential amplification reaction (HEAR) strategy that combines DNA strand displacement with a "who triggers, who gets generated" mode, providing excellent single-base discrimination and a reduced background signal. The detection limit is 19 aM, which is reduced by 3 orders of magnitude compared to traditional exponential amplification approaches. This one-pot strategy also exhibits a wide dynamic range, high specificity and short detection time. It is expected to become a powerful tool for clinical diagnosis.