A Continuous Add‐On Probe Reveals the Nonlinear Enlargement of Mitochondria in Light‐Activated Oncosis. Issue 17 (1st July 2021)

• Record Type: Journal Article
• Authors: Wang, Kang‐Nan; Shao, Xintian; Tian, Zhiqi; Liu, Liu‐Yi; Zhang, Chengying; Tan, Cai‐Ping; Zhang, Jie; Ling, Peixue; Liu, Fei; Chen, Qixin; Diao, Jiajie; Mao, Zong‐Wan
• Is Part Of: Advanced science. Volume 8:Issue 17(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-01
• Subjects: nonlinear enlargement of mitochondria -- oncosis -- continuous add‐on probe -- dual‐color imaging -- super‐resolution imaging; Science -- Periodicals; 505
• DOI: 10.1002/advs.202004566
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Oncosis, depending on DNA damage and mitochondrial swelling, is an important approach for treating cancer and other diseases. However, little is known about the behavior of mitochondria during oncosis, due to the lack of probes for in situ visual illumination of the mitochondrial membrane and mtDNA. Herein, a mitochondrial lipid and mtDNA dual‐labeled probe, MitoMN, and a continuous add‐on assay, are designed to image the dynamic process of mitochondria in conditions that are unobservable with current mitochondrial probes. Meanwhile, the MitoMN can induce oncosis in a light‐activated manner, which results in the enlargement of mitochondria and the death of cancer cells. Using structured illumination microscopy (SIM), MitoMN ‐stained mitochondria with a dual‐color response reveals, for the first time, how swelled mitochondria interacts and fuses with each other for a nonlinear enlargement to accelerate oncosis into an irreversible stage. With this sign of irreversible oncosis revealed by MitoMN, oncosis can be segregated into three stages, including before oncosis, initial oncosis, and accelerated oncosis. Abstract : A mitochondrial lipids and mtDNA dual‐labeling probe with continuous add‐on assay is designed to induce and image the nonlinear enlargement of mitochondria during oncosis. Together with super‐resolution imaging, it is revealed how swelled mitochondria interact and fuse with each other for a nonlinear enlargement to accelerate oncosis into an irreversible stage, which can be a new landmark for staging oncosis process.