Calcium–Collagen Coupling is Vital for Biomineralization Schedule. Issue 15 (27th May 2021)

• Record Type: Journal Article
• Authors: Zhang, Jinglun; Ji, Yaoting; Jiang, Shuting; Shi, Miusi; Cai, Wenjin; Miron, Richard J.; Zhang, Yufeng
• Is Part Of: Advanced science. Volume 8:Issue 15(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-27
• Subjects: biomineralization -- bones -- bone biomaterials -- calcium–collagen coupling -- collagen; Science -- Periodicals; 505
• DOI: 10.1002/advs.202100363
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Biomineralization is a chemical reaction that occurs in organisms in which collagen initiates and guides the growth and crystallization of matched apatite minerals. However, there is little known about the demand pattern for calcium salts and collagen needed by biomineralization. In this study, natural bone biomineralization is analyzed, and a novel interplay between calcium concentration and collagen production is observed. Any quantitative change in one of the entities causes a corresponding change in the other. Translocation‐associated membrane protein 2 (TRAM2) is identified as an intermediate factor whose silencing disrupts this relationship and causes poor mineralization. TRAM2 directly interacts with the sarcoplasmic/endoplasmic reticulum calcium ATPase 2b (SERCA2b) and modulates SERCA2b activity to couple calcium enrichment with collagen biosynthesis. Collectively, these findings indicate that osteoblasts can independently and directly regulate the process of biomineralization via this coupling. This knowledge has significant implications for the developmentally inspired design of biomaterials for bone regenerative applications. Abstract : This study clarifies the biological demand pattern of osteoblasts for ER Ca 2+ concentration and collagen biosynthesis and demonstrates that the translocation‐associated membrane protein 2‐sarcoplasmic/endoplasmic reticulum calcium ATPase 2b (TRAM2‐SERCA2b) regulatory complex can mediate the two‐way relationship during biomineralization. The synchronicity of ER morphology, TRAM2 expression patterns, and SERCA2b activity trends reveal the central position of ER during the entire process of biomineralization.