Transcriptome analysis reveals self-redox mineralization mechanism of azo dyes and novel decolorizing hydrolases in Aspergillus tabacinus LZ-M. (15th May 2023)

• Record Type: Journal Article
• Authors: Yu, Xuan; Mao, Chunlan; Zong, Simin; Khan, Aman; Wang, Wenxue; Yun, Hui; Zhang, Peng; Shigaki, Toshiro; Fang, Yitian; Han, Huawen; Li, Xiangkai
• Is Part Of: Environmental pollution. Volume 325(2023)
• Publication Date: 2023-05-15
• Subjects: Azo dyes -- Aspergillus -- Mechanism -- Decolorizing enzyme; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121459
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Bio-degradation is the most affordable method of azo dye decontamination, while its drawbacks such as aromatic amines accumulation and low degradation efficiency must be overcome. In this study, a novel mechanism of azo dye degradation by a fungus was discovered. At a concentration of 400 mg/L, the decolorization efficiency of Acid Red 73 (AR73) by Aspergillus tabacinus LZ-M was 90.28%. Metabolite analysis and transcriptome sequencing analysis revealed a self-redox process of AR73 degradation, where the electrons generated in carbon oxidation were transferred to the reduction of -C-N = and –NN. The metabolites, 2-hydroxynaphthalene and N-phenylnitrous amide were mineralized into CO2 through catechol pathway and a glycolytic process. Furthermore, the mineralization ratio of dye was computed to be 31.8% by the carbon balance and electron balance. By using comparative transcriptome, a novel decoloring enzyme Ord95 was discovered in unknown genes through gene cloning. It hydrolyzed AR73 into 2-hydroxynaphthalene and N-phenylnitrous amide, containing a glutathione S-transferase domain with three arginines as key active sites. Here the new mechanism of azo dye degradation was discovered with identification of a novel enzyme in Aspergillus tabacinus LZ-M. Graphical abstract: Image 1 Highlights: AcId red 73 (AR73) was mineralized by A. tabacinus LZ-M under anaerobic condition. The self-redox mechanism of AR73 degradation was revealed firstly. New hydrolase Ord95 was found for cleaving -C-N = in AR73 at initial step. Intermediate metabolites were degraded via catechol pathway and glycolysis process.