Advanced bile acid-based multi-compartmental microencapsulated pancreatic β-cells integrating a polyelectrolyte-bile acid formulation, for diabetes treatment. (17th February 2016)

• Record Type: Journal Article
• Authors: Mooranian, Armin; Negrulj, Rebecca; Chen-Tan, Nigel; Fakhoury, Marc; Arfuso, Frank; Jones, Franca; Al-Salami, Hani
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 44:Number 2(2016)
• Page Start: 588
• Page End: 595
• Publication Date: 2016-02-17
• Subjects: bile acid -- microencapsulation -- pancreatic beta cells -- Seahorse analyzer; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.3109/21691401.2014.971806
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract : This study utilized the Seahorse Analyzer to examine the effect of the bile acid ursodeoxycholic acid (UDCA), on the morphology, swelling, stability, and size of novel microencapsulated β-cells, in real-time. UDCA was conjugated with fluorescent compounds, and its partitioning within the microcapsules was examined using confocal microscopy. UDCA produced microcapsules with good morphology, better mechanical strength ( p < 0.01), and reduced swelling properties ( p < 0.01), but lower cell viability ( p < 0.05) and cell count per microcapsule ( p < 0.01). UDCA reduced the cells' biochemical activities, mitochondrial respiration, and energy production, post-microencapsulation. This is the first time biological functions of microencapsulated β-cells have been analyzed in real-time.