Synthesis of calcium phosphate-zirconia scaffold and human endometrial adult stem cells for bone tissue engineering. (2nd January 2016)

• Record Type: Journal Article
• Authors: Alizadeh, Aliakbar; Moztarzadeh, Fathollah; Ostad, Seyed Naser; Azami, Mahmoud; Geramizadeh, Bita; Hatam, Gholamreza; Bizari, Davood; Tavangar, Seyed Mohammad; Vasei, Mohammad; Ai, Jafar
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 44:Number 1(2016)
• Page Start: 66
• Page End: 73
• Publication Date: 2016-01-02
• Subjects: β-tricalcium phosphate -- bone tissue engineering -- endometrial stem cells -- porous scaffold -- zirconia; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.3109/21691401.2014.909825
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract : To address the hypothesis that using a zirconia (ZrO2 )/ β-tricalcium phosphate (β-TCP) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO2 /β-TCP composite scaffolds with different ZrO2 /β-TCP ratios, and evaluated their physical and mechanical characteristics, also the effect of three-dimensional (3D) culture (ZrO2 /β-TCP scaffold) on the behavior of human endometrial stem cells. Results showed the porosity of a ZrO2 /β-TCP scaffold can be adjusted from 65% to 84%, and the compressive strength of the scaffold increased from 4.95 to 6.25 MPa when the ZrO2 content increased from 30 to 50 wt%. The cell adhesion and proliferation in the ZrO2 /β-TCP scaffold was greatly improved when ZrO2 decreased. Moreover, in vitro study showed that an osteoblasts-loaded ZrO2 /β-TCP scaffold provided a suitable 3D environment for osteoblast survival and enhanced bone regeneration. We thus showed that a porous ZrO2 /β-TCP composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed during in vivo study for treatment of large bone defects.