Characterization of reactive intermediates formation in dacomitinib metabolism and bioactivation pathways elucidation by LC-MS/MS: in vitro phase I metabolic investigation. Issue 68 (19th November 2018)

• Record Type: Journal Article
• Authors: Attwa, Mohamed W.; Kadi, Adnan A.; Abdelhameed, Ali S.
• Is Part Of: RSC advances. Volume 8:Issue 68(2018)
• Page Start: 38733
• Page End: 38744
• Publication Date: 2018-11-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra06709k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Dacomitinib (DCB) is a second generation irreversible tyrosine kinase inhibitor (TKI) that is claimed to overcome the disadvantages of the resistance developed by the first line epidermal growth factor receptor (EGFR) TKIs. Abstract : Dacomitinib (DCB) is a second generation irreversible tyrosine kinase inhibitor (TKI) that is claimed to overcome the disadvantages of the resistance developed by the first line epidermal growth factor receptor (EGFR) TKIs. In the current study, metabolites of phase I for DCB were systematically explored. DCB reactive metabolites were also investigated in rat liver microsomes in presence of potassium cyanide or methoxylamine that were employed as capturing agents for iminium reactive intermediates and aldehyde, respectively, to form stable complexes which can be detected by LC-MS/MS. As a result, four in vitro phase I metabolites were observed with major pathway of piperidine ring hydroxylation. Additionally, two potentially reactive intermediates, one aldehyde and one iminium ions were characterized. Two different pathways of bioactivation were ultimately proposed.