RIP140/PGC-1α axis involved in vitamin A-induced neural differentiation by increasing mitochondrial function. (31st October 2018)

• Record Type: Journal Article
• Authors: Mu, Qing; Yu, Weidong; Zheng, Shuying; Shi, Hongxia; Li, Mei; Sun, Jie; Wang, Di; Hou, Xiaoli; Liu, Ling; Wang, Xinjuan; Zhao, Zhuran; Liang, Rong; Zhang, Xue; Dong, Wei; Zeng, Chaomei; Guo, Jingzhu
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 46(2018)Supplement 1
• Page Start: 806
• Page End: 816
• Publication Date: 2018-10-31
• Subjects: Vitamin A deficiency -- retinoic acid -- retinoic acid receptors -- receptor-interacting protein-140 -- neural differentiation -- mitochondrial dysfunction -- nuclear-encoded mitochondrial genes (NEMGs) -- peroxisome proliferator-activated receptor-γ co-activator 1-α (PGC-1α); Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2018.1436552
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: Vitamin A deficiency and mitochondrial dysfunction are both associated with neural differentiation-related disorders, such as Alzheimer's disease (AD) and Down syndrome (DS). The mechanism of vitamin A-induced neural differentiation and the notion that vitamin A can regulate the morphology and function of mitochondria in its induction of neural differentiation through the RIP140/PGC-1α axis are unclear. The aim of this study was to investigate the roles and underlying mechanisms of RIP140/PGC-1α axis in vitamin A-induced neural differentiation. Human neuroblastoma cells (SH-SY5Y) were used as a model of neural stem cells, which were incubated with DMSO, 9- cis -retinoic acid (9- cis -RA), 13- cis -retinoic acid (13- cis -RA) and all- trans -retinoic acid (at-RA). Neural differentiation of SH-SY5Y was evaluated by Sandquist calculation, combined with immunofluorescence and real-time polymerase chain reaction (PCR) of neural markers. Mitochondrial function was estimated by ultrastructure assay using transmission electron microscopy (TEM) combined with the expression of PGC-1α and NEMGs using real-time PCR. The participation of the RA signaling pathway was demonstrated by adding RA receptor antagonists. Vitamin A derivatives are able to regulate mitochondrial morphology and function, and furthermore to induce neural differentiation through the RA signaling pathway. The RIP140/PGC-1α axis is involved in the regulation of mitochondrial function in vitamin A derivative-induced neural differentiation.