Naturally derived dual dynamic crosslinked multifunctional hydrogel for diabetic wound healing. (15th May 2023)

• Record Type: Journal Article
• Authors: Shi, Tongtong; Lu, Hanzhi; Zhu, Jianyong; Zhou, Xiaojun; He, Chuanglong; Li, Fulun; Yang, Guang
• Is Part Of: Composites. Number 257(2023)
• Publication Date: 2023-05-15
• Subjects: Multifunctional hydrogel -- Keratin -- Dual dynamic crosslinking -- Diabetic wound; Composite materials -- Periodicals; Materials science -- Periodicals; Composite materials; Periodicals; Electronic journals; 620.118
• DOI: 10.1016/j.compositesb.2023.110687
• Languages: English
• ISSNs: 1359-8368

Abstract

Abstract: Diabetic wound treatments remain a significant challenge for regenerative medicine due to the unicity of clinical dressings, which lack systemic multifunction with injectability, self-healing ability, moderate tissue adhesion, anti-inflammation and antibacterial abilities, and guidance of tissue repair. Herein, a naturally derived multifunctional hydrogel dressing composed of keratin, protocatechuic aldehyde (PA) and iron ions is fabricated through a simple mixing process, circumventing the use of any extra crosslinking agents or functional modification. Our findings showed that a dual dynamic crosslinked network was constructed through a dynamic thiol-aldehyde addition reaction between thiol groups in keratin and aldehyde groups in PA, and catechol-metal ions coordination bonds between catechol groups in PA and Fe 3+, and realized good injectability, mechanical performance, self-healing ability, tissue adhesion and antibacterial properties of the hydrogel. Moreover, the incorporation of phellopterin (PP), a natural compound derived from traditional Chinese herb Angelica dahurica endowed the hydrogel with anti-inflammation properties. The PP-loaded hydrogel shortened inflammation duration and accelerated wound repair in the full-thickness wounds of streptozotocin-induced diabetic mice, notably by promoting macrophage polarization towards anti-inflammatory phenotype and resolving local inflammation. The PP-loaded hydrogel achieved almost wound closure after only 10 days for a diabetic wound of 6 mm in diameter. This study demonstrated that the PP-loaded dynamic crosslinked multifunctional hydrogel had a great potential in the treatment of diabetic wounds. Graphical abstract: Image 1 Highlights: The unicity of clinical dressings limits the treatment of diabetic wounds. A naturally derived multifunctional hydrogel was fabricated in a simple way. A dual dynamic crosslinked network was constructed in the hydrogel. Injectability, self-healing, adhesion and antibacterial ability was achieved. Phellopterin-loaded hydrogel showed anti-inflammation property.