Nano-selenium protects grass carp hepatocytes against 4-tert-butylphenol-induced mitochondrial apoptosis and necroptosis via suppressing ROS-PARP1 axis. Issue 135 (April 2023)

• Record Type: Journal Article
• Authors: Cui, Jiawen; Qiu, Minna; Liu, Yuhang; Liu, Yuhao; Tang, You; Teng, Xiaohua; Li, Shu
• Is Part Of: Fish & shellfish immunology. Issue 135(2023)
• Publication Date: 2023-04
• Subjects: Nano-Se -- Grass carp -- Hepatocytes -- Apoptosis -- Necroptosis; EDCs endocrine disrupting chemicals -- 4-tBP 4-tert-butylphenol -- ROS reactive oxygen species -- Nac N-Acetylcysteine -- Se Selenium -- Nec-1 Necrostatin-1 -- 3-AB 3-Aminobenzamide -- PARP1 poly(ADP-ribose) polymerase 1 -- AV/PI Annexin V-FITC/PI -- AO/EB Acridine orange/ethidium bromide -- MMP Mitochondrial membrane potential -- RIPK1 receptor interacting serine/threonine kinase 1 -- BSA bovine serum albumin -- qRT-PCR Real-time quantitative PCR -- Bid BH3 interacting domain death agonist -- TNF-α tumour necrosis factor alpha -- TNFR1 TNF receptor superfamily member 1A -- Bax Bcl-2 associated X -- AIFM1 apoptosis inducing factor mitochondria associated 1 -- CytC cytochrome C -- APAF1 apoptotic peptidase activating factor 1 -- RIPK3 receptor interacting serine/threonine kinase 3 -- MLKL mixed lineage kinase domain like pseudokinase; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108682
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: 4-tert-butylphenol (4-tBP) is a monomer widely used in the synthesis of industrial chemicals, and posed a high risk to aquatic animals. Our study focused on toxic phenotype and mechanism of detoxification in grass carp hepatocytes (L8824) after 4-tBP-treatment. In this experiment, L8824 displayed hallmark phenotypes of apoptosis and necroptosis after 4-tBP exposure, as evidenced by changes in cell morphology, increased rates of apoptosis and necrosis, the loss of MMP, the accumulation of ROS, and changes in associated factors (PARP1, JNK, Bid, Bcl-2, Bax, AIFM1, CytC, Caspase 9, APAF1, Caspase 3, TNF-α, TNFR1, RIPK1, RIPK3, and MLKL). Furthermore, we found that 4-tBP-induced apoptosis and necroptosis were reversed by pretreating with N-Acetylcysteine (a ROS scavenger) and 3-Aminobenzamide (a PARP1 inhibitor), indicating that 4-tBP induced the onset of mitochondrial apoptosis and necroptosis in L8824 via activating ROS-PARP1 axis. Nano-selenium (Nano-Se) is a novel form of Se with a noteworthy antioxidant capacity. Here, Nano-Se was found to have preventive, therapeutic, and resistance effects on 4-tBP-induced L8824 apoptosis and necroptosis. Nano-Se co-treatment with 4-tBP was an optimal way to alleviate 4-tBP-induced apoptosis and necroptosis. We demonstrated for the first time that Nano-Se protected L8824 against 4-tBP-induced mitochondrial apoptosis and necroptosis through ROS-PARP1 pathway. This study will provide a new theoretical basis for 4-tBP toxicology researches and aquatic animal protection. Graphical abstract: Image 1 Highlights: Endocrine disrupting chemical 4-tBP induced apoptosis and necroptosis in L8824. Nano-Se can prevent, treat, and resist 4-tBP-induced apoptosis and necroptosis. The optimal relief effect was achieved by Nano-Se co-treatment with 4-tBP. Nano-Se improved 4-tBP-induced apoptosis and necroptosis via ROS-PARP1 pathway.