Validated high‐performance thin‐layer chromatographic method for quantification of gallic acid and ellagic acid in fruits of Terminaliachebula, Phyllanthusemblica, and Quercusinfectoria. Issue 6 (9th February 2023)

• Record Type: Journal Article
• Authors: Godiyal, Shilpa; Laddha, Kirti
• Is Part Of: Journal of separation science. Volume 46:Issue 6(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-02-09
• Subjects: gallic acid -- high‐performance thin‐layer chromatography -- Phyllanthus emblica -- Quercus infectoria -- Terminalia chebula; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.202200991
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : A simple and rapid high‐performance thin‐layer chromatographic method for quantification of gallic acid and ellagic acid in dried fruits of Terminalia chebula, Phyllanthus emblica, and Quercus infectoria has been developed. The chromatographic development was carried out on precoated silica gel 60 F254 plates in a mixture of toluene:ethyl acetate:chloroform:formic acid (4:8:1:3 v/v/v/v). The plate was scanned densitometrically at a wavelength of 280 nm. The retention factor value of gallic acid and ellagic acid was found to be 0.63 ± 0.2 and 0.53 ± 0.1, respectively. The developed method was validated in terms of linearity, precision, accuracy, sensitivity, robustness, specificity and stability as per the international conference of harmonization guidelines. The method showed good linear relationship over a range of 100–600 ng/band (gallic acid) and 100–500 ng/band (ellagic acid) with a regression coefficient ( r 2 ) of 0.997 (gallic acid) and 0.996 (ellagic acid). The method showed high accuracy (99.65%–100.85%). The percentage relative standard deviation of intra‐day and inter‐day precision studies was not more than 2%. The method is highly robust and has displayed high specificity. The developed method is new, simple, and accurate and can be successfully employed in routine analysis of raw materials and formulations containing gallic acid and ellagic acid.