Decapentaplegic retards lipolysis during metamorphosis in Bombyx mori and Drosophila melanogaster. (April 2023)

• Record Type: Journal Article
• Authors: Qian, Wenliang; Guo, Mengge; Peng, Jian; Zhao, Tujing; Li, Zheng; Yang, Yan; Li, Hao; Zhang, Xing; King-Jones, Kirst; Cheng, Daojun
• Is Part Of: Insect biochemistry and molecular biology. Volume 155(2023)
• Publication Date: 2023-04
• Subjects: Dpp -- Fat body -- Lipolysis -- Lipolytic enzyme -- Metamorphosis; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2023.103928
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: Insect morphogen decapentaplegic (Dpp) functions as one of the key extracellular ligands of the Bone Morphogenetic Protein (BMP) signaling pathway. Previous studies in insects mainly focused on the roles of Dpp during embryonic development and the formation of adult wings. In this study, we demonstrate a new role for Dpp in retarding lipolysis during metamorphosis in both Bombyx mori and Drosophila melanogaster . CRISPR/Cas9-mediated mutation of Bombyx dpp causes pupal lethality, induces an excessive and premature breakdown of lipids in the fat body, and upregulates the expressions of several lipolytic enzyme genes, including brummer ( bmm ), lipase 3 ( lip3 ), and hormone-sensitive lipase ( hsl ), and lipid storage droplet 1 ( lsd1 ), a lipid droplets (LD)-associated protein gene. Further investigation in Drosophila reveals that salivary gland-specific knockdown of the dpp gene and fat body-specific knockdown of Mad involved in Dpp signaling phenocopy the effects of Bombyx dpp mutation on pupal development and lipolysis. Taken together, our data indicate that the Dpp-mediated BMP signaling in the fat body maintains lipid homeostasis by retarding lipolysis, which is necessary for pupa-adult transition during insect metamorphosis. Graphical abstract: Image 1 Highlights: The loss of Bombyx Dpp function disrupts the pupa-adult transition. Dpp retards lipolysis during Bombyx metamorphosis. Dpp regulation of lipolysis is conserved in Drosophila.