Apoptosis of type I spiral ganglion neuron cells in Otof-mutant mice. (23rd April 2023)

• Record Type: Journal Article
• Authors: Tsuzuki, Nobuyoshi; Namba, Kazunori; Saegusa, Chika; Mutai, Hideki; Nishiyama, Takanori; Oishi, Naoki; Matsunaga, Tatsuo; Fujioka, Masato; Ozawa, Hiroyuki
• Is Part Of: Neuroscience letters. Volume 803(2023)
• Publication Date: 2023-04-23
• Subjects: Inner hair cell -- Otoferlin -- OTOF -- Spiral ganglion neurons -- Apoptosis -- Auditory neuropathy spectrum disorder; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2023.137178
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Otof tm1a/tm1a mice showed a reduction in spiral ganglion neurons (SGNs). The reduction in SGNs was accompanied by apoptosis of type Ⅰ SGNs. Otof tm1a/tm1a mice did not show a reduction in type Ⅱ SGNs. Appropriate glutamatergic synaptic inputs may be important for the survival of SGNs. Abstract: Otof, which encodes otoferlin, knockout mice are considered model mice for auditory neuropathy spectrum disorder, which is characterized by an absent auditory brainstem response (ABR) despite preserved distortion product otoacoustic emission (DPOAE). Although otoferlin-deficient mice lack neurotransmitter release at the inner hair cell (IHC) synapse, it remains unclear how the Otof mutation affects spiral ganglions. Thus, we used Otof -mutant mice carrying the Otof tm1a(KOMP)Wtsi allele ( Otof tm1a ) and analyzed spiral ganglion neurons (SGNs) in Otof tm1a/tm1a mice by immunolabeling type Ⅰ SGNs (SGN-Ⅰ) and type II SGNs (SGN-II). We also examined apoptotic cells in SGNs. Four-week-old Otof tm1a/tm1a mice had an absent ABR but normal DPOAEs. The number of SGNs was significantly lower in Otof tm1a/tm1a mice on postnatal day 7 (P7), P14, and P28 compared with that of wild-type mice. Moreover, significantly more apoptotic SGNs were observed in Otof tm1a/tm1a mice than in wild-type mice on P7, P14, and P28. SGN-IIs were not significantly reduced in Otof tm1a/tm1a mice on P7, P14, and P28. No apoptotic SGN-IIs were observed under our experimental conditions. In summary, Otof tm1a/tm1a mice showed a reduction in SGNs accompanied by apoptosis of SGN-Ⅰs even before the onset of hearing. We speculate that the reduction in SGNs with apoptosis is a secondary defect caused by a lack of otoferlin in IHCs. Appropriate glutamatergic synaptic inputs may be important for the survival of SGNs.