Shrimp MANF maintains hemocyte viability via interaction with a tyrosine kinase Abl. (June 2023)

• Record Type: Journal Article
• Authors: Chen, Yaohui; Luo, Kaiwen; Zhang, Baoyuan; Lu, Zhiyao; Wang, Fan
• Is Part Of: Developmental and comparative immunology. Volume 143(2023)
• Publication Date: 2023-06
• Subjects: MANF -- ERK signaling -- Abl; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2023.104675
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a highly conserved cell protective protein. In this study, we explored its functions in shrimp hemocytes. Our results indicated that LvMANF knockdown could cause a decrease in total hemocyte count (THC) and an increase in caspase3/7 activity. To further explore its working mechanism, transcriptomic analyses were performed with wild-type and LvMANF-knockdown hemocytes. Three upregulated genes from transcriptomic data, including FAS-associated factor 2, rho-associated protein kinase 1, and serine/threonine-protein kinase WNK4 were validated with qPCR. Further experiments showed that LvMANF knockdown and tyrosine kinase LvAbl knockdown could decrease tyrosine phosphorylation in shrimp hemocytes. In addition, the interaction between LvMANF and LvAbl was validated with immunoprecipitation. The knockdown of LvMANF would decrease ERK phosphorylation and increase LvAbl expression. Our results suggest intracellular LvMANF may maintain shrimp hemocyte viability by interacting with LvAbl. Highlights: LvMANF keeps shrimp hemocyte viability. LvMANF maintains ERK-pathway activation in shrimp hemocyte. LvMANF affects shrimp hemocyte tyrosine phosphorylation. LvMANF interacts with a tyrosine kinase LvAbl.