Preferential arborization of dendrites and axons of parvalbumin- and somatostatin-positive GABAergic neurons within subregions of the mouse claustrum. (May 2023)

• Record Type: Journal Article
• Authors: Takahashi, Megumu; Kobayashi, Tomoyo; Mizuma, Haruhi; Yamauchi, Kenta; Okamoto, Shinichiro; Okamoto, Kazuki; Ishida, Yoko; Koike, Masato; Watanabe, Masahiko; Isa, Tadashi; Hioki, Hiroyuki
• Is Part Of: Neuroscience research. Volume 190(2023)
• Page Start: 92
• Page End: 106
• Publication Date: 2023-05
• Subjects: Adeno-associated virus -- Claustrum subregion -- Cre-lox recombination -- GABAergic -- Local circuit -- Single-neuron reconstruction -- Tissue clearing; Neurosciences -- Research -- Periodicals; Neurosciences -- Research -- Japan -- Periodicals; Neurology -- Periodicals; Neurosciences -- Periodicals; Neurosciences -- Recherche -- Périodiques; Neurosciences -- Recherche -- Japon -- Périodiques; Neurosciences -- Research; Japan; Periodicals; 612.8
• DOI: 10.1016/j.neures.2022.11.008
• Languages: English
• ISSNs: 0168-0102

Abstract

Abstract: The claustrum coordinates the activities of individual cortical areas through abundant reciprocal connections with the cerebral cortex. Although these excitatory connections have been extensively investigated in three subregions of the claustrum—core region and dorsal and ventral shell regions—the contribution of GABAergic neurons to the circuitry in each subregion remains unclear. Here, we examined the distribution of GABAergic neurons and their dendritic and axonal arborizations in each subregion. Combining in situ hybridization with immunofluorescence histochemistry showed that approximately 10% of neuronal nuclei-positive cells expressed glutamic acid decarboxylase 67 mRNA across the claustral subregions. Approximately 20%, 30%, and 10% of GABAergic neurons were immunoreactive for parvalbumin (PV), somatostatin (SOM), and vasoactive intestinal polypeptide, respectively, in each subregion, and these neurochemical markers showed little overlap with each other. We then reconstructed PV and SOM neurons labeled with adeno-associated virus vectors. The dendrites and axons of PV and SOM neurons were preferentially localized to their respective subregions where their cell bodies were located. Furthermore, the axons were preferentially extended in a rostrocaudal direction, whereas the dendrites were relatively isotropic. The present findings suggest that claustral PV and SOM neurons might execute information processing separately within the core and shell regions. Highlights: The distribution of GABAergic neurons is examined in claustral subregions. Nearly 10% of claustral neurons are GABAergic in each claustral subregion. PV-, SOM-, and VIP-positive neurons comprise 20%, 30%, and 10% of GABAergic neurons. PV and SOM neurons preferentially extend the axons along a rostrocaudal axis. PV and SOM neurons localize the neurites within either the core or shell subregion.