Differentially-expressed genes related to glutathione metabolism and heavy metal transport reveals an adaptive, genotype-specific mechanism to Hg2+ exposure in rice (Oryza sativa L.). (1st May 2023)

• Record Type: Journal Article
• Authors: Wang, Shufeng; Yao, Hesheng; Li, Lingyi; Du, Hongxia; Guo, Pan; Wang, Dingyong; Rennenberg, Heinz; Ma, Ming
• Is Part Of: Environmental pollution. Volume 324(2023)
• Publication Date: 2023-05-01
• Subjects: Mercury -- Accumulation -- Tolerance -- Antioxidant system -- Differentially expressed genes (DEGs); Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121340
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Rice consumption is an essential cause of mercury (Hg) exposure for humans in Asia. However, the mechanism of Hg transport and accumulation in rice plants ( Oryza sativa L.) remains unclear. Here, rice genotypes with contrasting Hg uptake and translocation abilities, i.e. H655 (high Hg-accumulator) and H767 (low Hg-accumulator), were selected from 261 genotypes. Through comparative physiological and transcriptome analyses, we investigated the processes responsible for the relationship between Hg accumulation, transport and tolerance. The results showed significant stimulation of antioxidative metabolism, particularly glutathione (GSH) accumulation, and up-regulated expression of regulatory genes of glutathione metabolism for H655, but not for H767. In addition, up-regulated expression of GSH S-transferase (GST) and OsPCS1 in H655 that catalyzes the binding of Hg and GSH, enhances the Hg detoxification capacity, while high-level expression of YSL2 in H655 enhances the transport ability for Hg. Conclusively, Hg accumulation in rice is a consequence of enhanced expression of genes related to Hg binding with GSH and Hg transport. With these results, the present study contributes to the selection of rice genotypes with limited Hg accumulation and to the mitigation of Hg migration in food chains thereby enhancing nutritional safety of Hg-polluted rice fields. Graphical abstract: Image 1 Highlights: High and low Hg-accumulators (H655 and H767) were screened from 261 rice genotypes. Antioxidant capacity of H655 in response to Hg stress was stronger than H767. Glutathione metabolism responded more exclusively to Hg stress in H655. Transporter contributed to Hg accumulation difference between H655 and H767.