ROS mediated pyroptosis-M1 polarization crosstalk participates in inflammation of chicken liver induced by bisphenol A and selenium deficiency. (1st May 2023)

• Record Type: Journal Article
• Authors: Shi, Xu; Xu, Tong; Li, Xiaojing; Sun, Xinyue; Zhang, Wenyue; Liu, Xiaojing; Wang, Yuqi; Zhang, Yilei; Xu, Shiwen
• Is Part Of: Environmental pollution. Volume 324(2023)
• Publication Date: 2023-05-01
• Subjects: BPA -- Se deficiency -- Chicken -- Liver inflammation -- Pyroptosis-M1 polarization crosstalk; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121392
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The earth's natural environmental factors and man-made industrial pollution often lead to the co-occurrence of environmental pathogenic factors and malnutrition. Bisphenol A (BPA) is a serious environmental endocrine disruptor, and its exposure can cause liver tissue damage. Selenium (Se) deficiency is a worldwide problem that afflicts thousands of people, and Se deficiency can cause M1/M2 imbalance. In addition, the crosstalk between hepatocyte and immune cell is closely related to the occurrence of hepatitis. Therefore, this study found for the first time that the combined exposure of BPA and Se deficiency caused liver pyroptosis and M1 polarization through ROS, and the crosstalk between pyroptosis and M1 polarization aggravated liver inflammation in chicken. In this study, the BPA or/and Se deficiency chicken liver, single and co-culture model of LMH and HD11 cells were established. The results displayed that BPA or Se deficiency induced liver inflammation accompanied by pyroptosis and M1 polarization through oxidative stress, and increased expressions of chemokines (CCL4, CCL17, CCL19, and MIF) and inflammatory factors (IL-1β and TNF-α). The vitro experiments further verified the above changes and showed that LMH pyroptosis promoted M1 polarization of HD11 cells, and vice versa. NAC counteracted pyroptosis and M1 polarization caused by BPA and low-Se, reducing the release of inflammatory factors. In brief, BPA and Se deficiency treatment can exacerbate liver inflammation by increasing oxidative stress to induce pyroptosis and M1 polarization. Graphical abstract: Image 1 Highlights: BPA or Se deficiency leads to liver pyroptosis and M1 polarization through oxidative stress. Combined exposure of BPA and Se deficiency has stronger liver injury than single treatment. ROS mediated pyroptosis-M1 polarization crosstalk amplifies liver inflammation.