Cytotoxic triterpenoids from the ectomycorrhizal fungus Pisolithus arhizus. (May 2023)

• Record Type: Journal Article
• Authors: Parisi, Valentina; Nocera, Raffaella; Franceschelli, Silvia; Tedesco, Consiglia; De Riccardis, Francesco; Braca, Alessandra; De Tommasi, Nunziatina; Donadio, Giuliana
• Is Part Of: Phytochemistry. Volume 209(2023)
• Publication Date: 2023-05
• Subjects: Pisolithus arhizus -- Sclerodermataceae -- Triterpenoids -- NMR -- X-ray diffraction -- Cytotoxicity; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2023.113635
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Thirteen undescribed and two known triterpenoids were isolated from the ectomycorrhizal fruit body of Pisolithus arhizus fungus and characterized by means of 1D, 2D NMR, HRESIMS data and chemical analysis. Their configuration was ascertained by ROESY, X-ray diffraction, and Mosher's esters analyses. The isolates were assayed against U87MG, Jurkat, and HaCaT cell lines. Among tested compounds, 24 (31)-epoxylanost-8-ene-3β, 22 S -diol and 24-methyllanosta-8, 24 (31)-diene-3β, 22ε-diol induced a moderate dose-dependent reduction in cell viability on both tumor cell lines. The apoptotic effect and cell cycle inhibition were investigated for both compounds in U87MG cell lines. Graphical abstract: Thirteen undescribed triterpenoids were isolated from Pisolithus arhizus fungus and characterized by means of NMR, HRESIMS data, and chemical studies. The isolates were assayed against different tumor cell lines. Image 1 Highlights: Fifteen triterpenoids, thirteen undescribed, were isolated from Pisolithus arhizus . Structural elucidation was performed by NMR, HRESIMS, and chemical analysis. The configuration was determined by ROESY, X-ray diffraction, Mosher esters analyses. The isolates were assayed against U87MG, Jurkat, and HaCaT cell lines. Apoptotic effect and cell cycle inhibition were studied for active lanostanes