In vivo DNA methylation editing in zebrafish. Issue 1 (31st December 2023)

• Record Type: Journal Article
• Authors: Liang, Fang; Dong, Zijiong; Ye, Jianmin; Hu, Wei; Bhandari, Ramji Kumar; Mai, Kangsen; Wang, Xuegeng
• Is Part Of: Epigenetics. Volume 18:Issue 1(2023)
• Publication Date: 2023-12-31
• Subjects: DNA methylation editing -- CRISPR/dCas9 -- Dnmt7 -- Tet2 -- Zebrafish; Epigenesis -- Periodicals; Epigenetica; 572.86505
• DOI: 10.1080/15592294.2023.2192326
• Languages: English
• ISSNs: 1559-2294

Abstract

ABSTRACT: The CRISPR/dCas9-based epigenome editing technique has driven much attention. Fused with a catalytic domain from Dnmt or Tet protein, the CRISPR/dCas9-DnmtCD or -TetCD systems possess the targeted DNA methylation editing ability and have established a series of in vitro and in vivo disease models. However, no publication has been reported on zebrafish ( Danio rerio ), an important animal model in biomedicine. The present study demonstrated that CRISPR/dCas9-Dnmt7 and -Tet2 catalytic domain fusions could site-specifically edit genomic DNA methylation in vivo in zebrafish and may serve as an efficient toolkit for DNA methylation editing in the zebrafish model.