The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata. Issue 5 (15th February 2022)

• Record Type: Journal Article
• Authors: Sun, Guiling; Xia, Mingzhang; Li, Jieping; Ma, Wen; Li, Qingzeng; Xie, Jinjin; Bai, Shenglong; Fang, Shanshan; Sun, Ting; Feng, Xinlei; Guo, Guanghui; Niu, Yanli; Hou, Jingyi; Ye, Wenling; Ma, Jianchao; Guo, Siyi; Wang, Hongliang; Long, Yu; Zhang, Xuebin; Zhang, Junli; Zhou, Hui; Li, Baozhu; Liu, Jiong; Zou, Changsong; Wang, Hai; Huang, Jinling; Galbraith, David W; Song, Chun-Peng
• Is Part Of: The Plant Cell. Volume 34:Issue 5(2022)
• Page Start: 1890
• Page End: 1911
• Publication Date: 2022-02-15
• DOI: 10.1093/plcell/koac047
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract: The unique morphology of grass stomata enables rapid responses to environmental changes. Deciphering the basis for these responses is critical for improving food security. We have developed a planta platform of single-nucleus RNA-sequencing by combined fluorescence-activated nuclei flow sorting, and used it to identify cell types in mature and developing stomata from 33, 098 nuclei of the maize epidermis-enriched tissues. Guard cells (GCs) and subsidiary cells (SCs) displayed differential expression of genes, besides those encoding transporters, involved in the abscisic acid, CO2, Ca 2+, starch metabolism, and blue light signaling pathways, implicating coordinated signal integration in speedy stomatal responses, and of genes affecting cell wall plasticity, implying a more sophisticated relationship between GCs and SCs in stomatal development and dumbbell-shaped guard cell formation. The trajectory of stomatal development identified in young tissues, and by comparison to the bulk RNA-seq data of the MUTE defective mutant in stomatal development, confirmed known features, and shed light on key participants in stomatal development. Our study provides a valuable, comprehensive, and fundamental foundation for further insights into grass stomatal function. Abstract : A cell atlas of maize peels and leaf bases created by single-nucleus RNA sequencing provides insights into the molecular basis of stomatal movement and development in grasses.