Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection. Issue 135 (April 2023)

• Record Type: Journal Article
• Authors: Liu, Houfu; Hu, Xiucai; Lian, Zhengyi; Luo, Zhang; Lv, Aijun; Tan, Jing
• Is Part Of: Fish & shellfish immunology. Issue 135(2023)
• Publication Date: 2023-04
• Subjects: Focal adhesion -- Cynoglossus semilaevis -- Vibrio vulnificus -- Vinculin; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108651
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Focal adhesion (FA) plays a key role in cell adhesion, migration and antibacterial immune, but it remained unclear in fish. In this study, half-smooth tongue sole Cynoglossus semilaevis were infected with Vibrio vulnificus, and then immune-related protein in the skin, especially for FA signaling pathway were screened and identified by iTRAQ analysis. Results showed that the differentially expressed proteins (DEPs) in skin immune response (eg., ITGA6, FN, COCH, AMBP, COL6A1, COL6A3, COL6A6, LAMB1, LAMC1, FLMNA) were firstly found in FA signaling pathway. Furthermore, the validation analysis of FA-related genes were basically consistent with the iTRAQ data at 36 hpi (r = 0.678, p < 0.01), and their spatio-temporal expressions were confirmed by qPCR analysis. The molecular characterization of vinculin of C. semilaevis was described. This study will provide a new perspective for understanding the molecular mechanism of FA signaling pathway in the skin immune response in marine fish. Highlights: FA signaling pathway in C. semilaevis upon V. vulnificus were screened and identified by iTRAQ analysis. Some DEPs in skin immune response were firstly found in FA signaling pathway. The spatio-temporal expression of FA-related genes was confirmed by qPCR analysis.