High‐Throughput, Label‐Free and Slide‐Free Histological Imaging by Computational Microscopy and Unsupervised Learning. Issue 2 (7th November 2021)

• Record Type: Journal Article
• Authors: Zhang, Yan; Kang, Lei; Wong, Ivy H. M.; Dai, Weixing; Li, Xiufeng; Chan, Ronald C. K.; Hsin, Michael K. Y.; Wong, Terence T. W.
• Is Part Of: Advanced science. Volume 9:Issue 2(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-11-07
• Subjects: computational microscopy -- histology -- label‐free imaging -- unsupervised learning; Science -- Periodicals; 505
• DOI: 10.1002/advs.202102358
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Rapid and high‐resolution histological imaging with minimal tissue preparation has long been a challenging and yet captivating medical pursuit. Here, the authors propose a promising and transformative histological imaging method, termed computational high‐throughput autofluorescence microscopy by pattern illumination (CHAMP). With the assistance of computational microscopy, CHAMP enables high‐throughput and label‐free imaging of thick and unprocessed tissues with large surface irregularity at an acquisition speed of 10 mm 2 /10 s with 1.1‐µm lateral resolution. Moreover, the CHAMP image can be transformed into a virtually stained histological image (Deep‐CHAMP) through unsupervised learning within 15 s, where significant cellular features are quantitatively extracted with high accuracy. The versatility of CHAMP is experimentally demonstrated using mouse brain/kidney and human lung tissues prepared with various clinical protocols, which enables a rapid and accurate intraoperative/postoperative pathological examination without tissue processing or staining, demonstrating its great potential as an assistive imaging platform for surgeons and pathologists to provide optimal adjuvant treatment. Abstract : A rapid, label‐free, and slide‐free histological imaging method, termed computational high‐throughput autofluorescence microscopy by pattern illumination (CHAMP) is proposed. With the assistance of computational imaging and unsupervised learning, CHAMP enables rapid and accurate pathological examination without tissue processing or staining, demonstrating great potential as an assistive imaging platform for surgeons and pathologists to provide optimal adjuvant treatment intraoperatively.