Mito-battery: Micro-nanohydrogel microspheres for targeted regulation of cellular mitochondrial respiratory chain. (April 2023)

• Record Type: Journal Article
• Authors: Wang, Xingkuan; Lei, Yiting; Jiang, Ke; Yan, Caiping; Shen, Jieliang; Zhao, Weikang; Xiang, Chao; Cai, Zhengwei; Song, Yang; Chen, Lu; Cui, Wenguo; Li, Yuling
• Is Part Of: Nano today. Volume 49(2023)
• Publication Date: 2023-04
• Subjects: Mitochondrial respiratory chain -- Hydrogel microsphere -- Microfluidics -- Injectable -- Osteoarthritis; Nanotechnology -- Periodicals; Nanosciences -- Périodiques; 620.505
• DOI: 10.1016/j.nantod.2023.101820
• Languages: English
• ISSNs: 1748-0132

Abstract

Abstract: The mitochondrial respiratory chain (MRC) plays an essential role in regulating cellular energy metabolism and oxidative stress. Repairing damaged MRC function can efficiently provide cells with energy to fight inflammation. Herein, with the inspiration by a rechargeable battery, microfluidics was used to prepare hyaluronic acid hydrogel microspheres as shells and con-covalently connected the inner core-SS-31 peptide and Wyrgrl peptide co-modified long-circulating liposomes loaded with resveratrol (REV) to the nanonetwork of microspheres to construct a "Mito-Battery" that could promote cell energy production and fight inflammation by targeted regulation of MRC functions. Modification of peptide could increase its cellular uptake and enhance the mitochondrial-targeting of "Mito-Battery." Meanwhile, REV loaded in the liposomes can activate SIRT3 protein and synergistically enhance the MRC electron transfer efficiency with SS-31. "Mito-Battery" effectively delayed the progression of the disease in a rat bone and joint model. Thus, "Mito-Battery" has great potential in the treatment of degenerative diseases related to MRC dysfunction. Graphical Abstract: Inspired by batteries provide energy through electronic transmission, micro-nano hydrogel microspheres for targeted regulation of cellular mitochondrial respiratory chain were engineered by microfluidic technology, it stabilizes the electron transfer efficiency of the mitochondrial respiratory chain, improves mitochondrial function, and provides energy to cells, like a biological energy battery. ga1 Highlights: Enhance the MRC electron transfer efficiency to therapy osteoarthritis via injectable Micro-nano hydrogel microsphere. The SS-31 and Wyrgrl peptide co-modified liposomes had high-efficiency targeting ability to chondrocytes mitochondria. The liposomes were anchored to the nano-network of microspheres through the charge dipolar effect. The micro-nano hydrogel microspheres promote the sustained release of resveratrol and maintain SS-31 peptide activity.