A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy. (6th March 2023)

• Record Type: Journal Article
• Authors: Wang, Xiaojing; Shepherd, Skye; Li, Nantao; Che, Congnyu; Song, Tingjie; Xiong, Yanyu; Palm, Isabella Rose; Zhao, Bin; Kohli, Manish; Demirci, Utkan; Lu, Yi; Cunningham, Brian T.
• Is Part Of: Angewandte Chemie. Volume 135:Number 16(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-03-06
• Subjects: Amplification -- Cancer Diagnostics -- DNA Toehold Probe -- Exosomal miRNA -- Photonic Sensor; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202217932
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis‐ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex digital detection with sub‐attomolar sensitivity in 20 minutes, robust selectivity for single nucleotide variants, and a broad dynamic range from 1 aM to 1 pM. Compared with traditional qRT‐PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but also exhibited at least 31‐fold and 61‐fold enhancement in the limits of miRNA‐375 and miRNA‐21 detection, respectively. The TRAP approach is ideal for exosomal or circulating miRNA biomarker quantification, where the miRNAs are present in low concentrations or sample volume, with potentials for frequent, low‐cost, and minimally invasive point‐of‐care testing. Abstract : A target recycling amplification process (TRAP) detection method was developed using photonic resonator absorption microscopy, which shows exciting potential for monitoring exosomal miRNAs with ultra‐sensitivity and single‐base mismatch selectivity. The approach is a single‐step, wash‐free, enzyme‐free, isothermal, 20‐minute, room temperature process that can be readily adapted toward any miRNA target through different DNA probe designs.