3D bioprinting of a gradient stiffened gelatin–alginate hydrogel with adipose-derived stem cells for full-thickness skin regeneration. Issue 13 (15th March 2023)

• Record Type: Journal Article
• Authors: Ma, Yuan; Wang, Yilin; Chen, Danni; Su, Ting; Chang, Qiang; Huang, Wenhua; Lu, Feng
• Is Part Of: Journal of materials chemistry. Volume 11:Issue 13(2023)
• Page Start: 2989
• Page End: 3000
• Publication Date: 2023-03-15
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb02200a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : 3D bioprinting of a gradient-stiffened gelatin–alginate hydrogel accelerated wound healing by promoting the proliferation, migration, and paracrine secretion of encapsulated ADSCs. Abstract : Current hydrogel-based scaffolds offer a promising approach to accelerate tissue regeneration, but great challenges remain in developing platforms that mimic the physical microenvironment of tissues combined with therapeutic biological cues. Here, a 3D bioprinting gelatin–alginate hydrogel for the construction of gradient composite scaffolds that mimic the dermal stiffness microenvironment was developed for architecture construction by extruding the bioink on calcium-containing substrates to achieve gradient secondary cross-linking, meanwhile, adipose-derived stem cells were encapsulated in the present hydrogels for therapeutic purposes. The gradient-stiffness scaffold exhibited good stability and biocompatibility as well as enhanced proliferation and migration of the adipose-derived stem cells. In addition, the promoted angiogenesis and healing efficiency was demonstrated via the animal wound model and was mainly attributed to the enhanced paracrine secretion of adipose-derived stem cells by the physical microenvironment provided within the gradient stiffness scaffold. The current 3D printed gradient scaffolds provide adipose-derived stem cells with a distinct yet successive architecture rather than the typical uniform microenvironment to accelerate skin regeneration, which may have broader applications in other chronic wounds or tissue defects.