Paenibacillus auburnensis sp. nov. and Paenibacillus pseudetheri sp. nov., isolated from the rhizosphere of Zea mays. Issue 4 (4th April 2023)

• Record Type: Journal Article
• Authors: Kämpfer, Peter; Lipski, André; McInroy, John A.; Clermont, Dominique; Lamothe, Lucie; Glaeser, Stefanie P.; Criscuolo, Alexis
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-04
• Subjects: endophyte -- Paenibacillus pseudetheri -- Paenibacillus auburnensis -- plant associated -- taxonomy -- Zea mays; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005808
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two Gram-stain-positive, aerobic, endospore-forming bacterial strains, isolated from the rhizosphere of Zea mays were studied for their detailed taxonomic allocation. Based on 16S rRNA gene sequence similarity comparisons, both strains JJ-7 T and JJ-60 T were shown to be members of the genus Paenibacillus . Strain JJ-7 T was most closely related to the type strains of Paenibacillus tianjinensis (99.6 %) and P. typhae (98.7 %), and strain JJ-60 T to Paenibacillus etheri (99.5 %). The 16S rRNA gene sequence similarities to all other Paenibacillus species were ≤98.4 %. Both strains JJ-7 T and JJ-60 T showed 97.6 % 16S rRNA gene sequence similarity between each other. Genomic comparisons showed that the average nucleotide identity and digital DNA–DNA hybridization values to next related type strain genomes were always <94 and <56 %, respectively. The polar lipid profiles of both strains contain a number of phospholipids including diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, which is in accord with the genus Paenibacillus . The major quinone was MK-7 in both strains. Major fatty acids were iso- and anteiso-branched. Physiological and biochemical characteristics allowed a further phenotypic differentiation of strains JJ-7 T and JJ-60 T from the most closely related species. Thus, each strain represents a novel species of the genus Paenibacillus, for which the names Paenibacillus auburnensis sp. nov. and Paenibacillus pseudetheri sp. nov. are proposed, with JJ-7 T (=CIP 111892 T =DSM 111785 T =LMG 32088 T =CCM 9087 T ) and JJ-60 T (=CIP 111894 T =DSM 111787 T =LMG 32090 T =CCM 9086 T ) as the type strains, respectively.