Effects of exogenous calcium on cadmium accumulation in amaranth. (June 2023)

• Record Type: Journal Article
• Authors: Xie, Hong; Liao, Zisheng; Li, Jun; Yang, Yongqiang; Chen, Fanrong; Zhu, Runliang; Xiang, Li; Wu, Shijun
• Is Part Of: Chemosphere. Volume 326(2023)
• Publication Date: 2023-06
• Subjects: Calcium oxalate (CaOx) crystals -- Cadmium -- Amaranthus tricolor L. -- Heavy metal detoxification; CaOx calcium oxalate -- HMs heavy metals -- BCF bioconcentration factor -- TF translocation factor; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2023.138435
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Calcium oxalate (CaOx) crystals in plants act as a sink for excess Ca and play an essential role in detoxifying heavy metals (HMs). However, the mechanism and related influencing factors remain unclear. Amaranth ( Amaranthus tricolor L.) is a common edible vegetable rich in CaOx and a potential Cd hyperaccumulation species. In this study, the hydroponic experiment was carried out to investigate the effect of exogenous Ca concentrations on Cd uptake by amaranth. The results showed that either insufficient or excess Ca supply inhibited amaranth growth, while the Cd bioconcentration factor (BCF) increased with Ca concentration. Meanwhile, the sequence extraction results demonstrated that Cd mainly accumulated as pectate and protein-bound species (NaCl extracted) in the root and stem, compared to pectate, protein, and phosphate-bound (acetic acid extractable) species in the leaf. Correlation analysis showed that the concentration of exogenous Ca was positively correlated with amaranth-produced CaOx crystals but negatively correlated with insoluble oxalate-bound Cd in the leaf. However, since the accumulated insoluble oxalate-bound Cd was relatively low, Cd detoxification via the CaOx pathway in amaranth is limited. Graphical abstract: Image 1 Highlights: Insufficient and excess Ca inhibited amaranth growth. The NaCl-extracted Cd dominated all organs of amaranth. The CaOx-bound Cd in leaves was significantly higher than in roots and stems. Excessive Ca promoted Cd bioconcentration while reducing CaOx-bound Cd accumulation. CaOx is not the dominant Cd detoxification pathway in amaranth.