The kisspeptin-GnIH signaling pathway in the role of zebrafish courtship and aggressive behavior induced by azoxystrobin. (15th May 2023)

• Record Type: Journal Article
• Authors: Guo, Xuanjun; Zhang, Ruihua; Jin, Qian; Cao, Niannian; Shi, Jingjing; Zong, Xingxing; Chen, Xuejun; Wang, Chen; Li, Xuefeng; Pang, Sen; Li, Liqin
• Is Part Of: Environmental pollution. Volume 325(2023)
• Publication Date: 2023-05-15
• Subjects: Azoxystrobin -- Zebrafish -- Social behavior -- GnRH -- 5-HT; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121461
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Azoxystrobin, a strobilurin widely used to control rice diseases, has raised concerns about possible adverse effects on aquatic ecosystems. At present, very little is known about the effects of azoxystrobin on courtship and aggressive behavior and the potential underlying mechanisms. In the present study, after exposing adult male and female zebrafish to worst-case scenario concentrations of azoxystrobin (0, 2 μg/L, 20 μg/L, and 200 μg/L) for 42 d, we observed a decrease in courtship behavior and an increase in aggressive behavior in both male and female zebrafish. In addition, to elucidate the molecular mechanism of the behavioral effects of azoxystrobin, we quantified the changes in the concentrations of kisspeptin, 5-HT, GnIH, and their corresponding receptor mRNA expression in the brain. The results showed that 200 μg/L azoxystrobin decreased the concentrations of kisspeptin and increased the concentration of GnIH in both male and female zebrafish brain. In addition, azoxystrobin also significantly reduced 5-HT concentration in female zebrafish brain. Further investigation revealed that altered courtship and aggressive behavior were associated with the expression levels of genes (kiss1, kiss2, gnrh3, gnrhr3, 5ht1a, and 5ht2a) involved in kisspeptin-GnIH signaling pathway. In conclusion, our study suggested that azoxystrobin may impair courtship and aggressive behavior in zebrafish by interfering with the kisspeptin-GnIH signaling pathway, which may have more profound effects on natural zebrafish populations. Graphical abstract: Image 1 Highlights: Azoxystrobin exposure decreased courtship behavior of male and female zebrafish. Azoxystrobin exposure increased aggressive behavior of male and female zebrafish. Azoxystrobin exposure disrupted kisspeptin-GnIH signaling pathway in zebrafish. Azoxystrobin exposure inhibited the expression of 5-HT-related receptors.