Identification of Suppressors of mbk-2/DYRK by Whole-Genome Sequencing. Issue 2 (1st February 2014)

• Record Type: Journal Article
• Authors: Wang, Yuemeng; Wang, Jennifer T; Rasoloson, Dominique; Stitzel, Michael L; O' Connell, Kevin F; Smith, Harold E; Seydoux, Geraldine
• Is Part Of: G3. Volume 4:Issue 2(2014)
• Page Start: 231
• Page End: 241
• Publication Date: 2014-02-01
• Subjects: whole-genome sequencing -- single nucleotide polymorphism mapping -- suppressors -- DYRK kinase -- MBK-2 -- C. elegans; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.113.009126
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: Screening for suppressor mutations is a powerful method to isolate genes that function in a common pathway or process. Because suppressor mutations often do not have phenotypes on their own, cloning of suppressor loci can be challenging. A method combining whole-genome sequencing (WGS) and single nucleotide polymorphism (SNP) mapping (WGS/SNP mapping) was developed to identify mutations with visible phenotypes in C. elegans . We show here that WGS/SNP mapping is an efficient method to map suppressor mutations without the need for previous phenotypic characterization. Using RNA-mediated interference to test candidate loci identified by WGS/SNP mapping, we identified 10 extragenic and six intragenic suppressors of mbk-2, a DYRK family kinase required for the transition from oocyte to zygote. Remarkably, seven suppressors are mutations in cell-cycle regulators that extend the timing of the oocyte-to-zygote transition.