Systematic engineering of Escherichia coli for efficient production of nicotinamide riboside from nicotinamide and 3-cyanopyridine. (June 2023)

• Record Type: Journal Article
• Authors: Huang, Zhongshi; Wang, Xinglong; Li, Ning; Song, Fuqiang; Zhou, Jingwen
• Is Part Of: Bioresource technology. Volume 377(2023)
• Publication Date: 2023-06
• Subjects: Nicotinamide riboside -- 3-cyanopyridine -- Metabolic engineering -- Escherichia coli -- Nitrile hydratase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2023.128953
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Biosynthesis of nicotinamide riboside (NR) from 3-cyanopyridine has been achieved. The function of UshA was confirmed and used for efficient biosynthesis of NR. Systematic engineering of NR-related pathway facilitates its accumulation. Fine-tuning of pathways and culture conditions increase NR titer to 29.8 g/L. Abstract: Nicotinamide riboside (NR), a key biosynthetic precursor of NAD +, is receiving increasing attention because of its role. In this study, a whole-cell catalysis method to efficiently synthesize NR was established. First, the performance of 5ʹ-nucleotidase (UshA) from Escherichia coli was confirmed to have high catalytic activity to synthesize NR. Then, the endogenous NR degradation pathway was detected, and the genes ( rihA, rihB, and rihC ) involved in NR degradation were knocked out, which enabled NR biosynthesis. In addition, the important role of the signal peptide of UshA in NR transport had been confirmed. Subsequently, nitrile hydratase was introduced to achieve the conversion of 3-cyanopyridine to NR. Finally, the NR titer reached 25.6 and 29.8 g/L with nicotinamide and 3-cyanopyridine, respectively, as substrates in a 5-L bioreactor, the efficient biosynthesis of NR in E. coli by using nicotinamide and 3-cyanopyridine.