Mass Spectrometry Proteomic Analysis of Four p53 Patterns in Differentiated Vulvar Intraepithelial Neoplasia. Issue 2 (6th April 2023)

• Record Type: Journal Article
• Authors: Jamaluddin, M. Fairuz B.; Day, Tania; Tanwar, Pradeep S.; Marzol, Alexandra; Scurry, James
• Is Part Of: Journal of lower genital tract disease. Volume 27:Issue 2(2023)
• Page Start: 146
• Page End: 151
• Publication Date: 2023-04-06
• Subjects: differentiated vulvar intraepithelial neoplasia -- vulva -- p53 -- immunohistochemistry -- mass spectrometry -- proteomics; Generative organs, Female -- Diseases -- Periodicals; Cervix uteri -- Diseases -- Periodicals; Generative organs -- Diseases -- Periodicals; Sexually transmitted diseases -- Periodicals; 618.1
• DOI: 10.1097/LGT.0000000000000720
• Languages: English
• ISSNs: 1089-2591

Abstract

Abstract : Different p53 immunohistochemistry patterns in differentiated vulvar intraepithelial neoplasia correspond to varied proteomic profiles. Abstract : Objective: The histopathologic diagnostic criteria of differentiated vulvar intraepithelial neoplasia (dVIN), the precursor of human papillomavirus-independent squamous cell carcinoma, are basal atypia, a negative or non–block-positive p16, and a supportive p53 immunohistochemistry (IHC). Several different patterns of supportive p53 IHC have been described. This study aims to determine the relationship between p53 IHC patterns and mass spectrometry analysis of cellular proteins in dVIN. Methods: Four patterns of p53 IHC were studied: overexpression, cytoplasmic, wild type, and intermediate expression between wild type and overexpression. For each pattern, tissue samples of 4 examples were subjected to mass spectrometry. Results: The protein profile within each p53 IHC pattern shared common features. Each of the 4 p53 patterns had a distinguishable protein profile when compared with the other 3 patterns. Conclusions: The distinguishable protein profiles in different p53 IHC patterns suggest diverse mechanisms of TP53 dysfunction. Subtyping dVIN by p53 IHC is worthy of further study because varied protein expression profiles may translate into different clinical behavior.