Nano-magnetic aptamer sensor incorporating AND logic recognition-launched hybridization chain reaction for organ origin identification of circulating tumor cells. (April 2023)

• Record Type: Journal Article
• Authors: Zhang, Yujia; Yu, Yue; Kang, Ke; Wang, Xuekang; Zhu, Nanhang; Wang, Xingyou; Yi, Qiangying; Wu, Yao
• Is Part Of: Nano today. Volume 49(2023)
• Publication Date: 2023-04
• Subjects: Circulating tumor cells -- Nano-magnetic aptamer sensor -- AND logic recognition -- Hybridization chain reaction -- Organ origin identification; Nanotechnology -- Periodicals; Nanosciences -- Périodiques; 620.505
• DOI: 10.1016/j.nantod.2023.101817
• Languages: English
• ISSNs: 1748-0132

Abstract

Abstract: Circulating tumor cells (CTCs) are effective bio-markers for early diagnosis and disease monitoring of cancer, but there is lack of simple and effective strategy to achieve the detection of multiple markers on CTCs while enriching, especially the identification of organ origin, limits their broad clinical applications. Here we present a nano-magnetic aptamer sensor platform to capture broad spectrum of epithelial CTCs and simultaneously identify their multiple organ origins by AND logic recognition-launched hybridization chain reaction. The platform exhibited excellent capture and enrichment capabilities for epithelial CTCs, and showed the ability to precisely identify their organ origin markers (GPC3 for liver and TTF1 for lung), even able to trace metastatic cancer to related organs. The developed nano-magnetic aptamer sensor platform provides a feasible strategy for multi-marker accurate detection of CTCs and a promising auxiliary tool for the precise treatment of cancer. Graphical Abstract: We developed a nano-magnetic aptamer sensor platform for capture and organ origin identification of circulating tumor cells (CTCs) using AND logic recognition-launched hybridization chain reaction. The proposed strategy can efficiently capture CTCs and identified subpopulation of CTCs, offering a new avenue for precise diagnosis of cancer. ga1 Highlights: Nano-magnetic aptamer sensor was fabricated for capture and organ origin identification of CTCs simultaneously. AND logic recognition-launched hybridization chain reactions amplified organ-associated phenotypic signals. The proposed could precisely identify organ origin of CTCs, even able to trace metastatic cancer to related organs.