The role of multi-low molecular weight organic acids on phenanthrene biodegradation: Insight from cellular characteristics and proteomics. (June 2023)

• Record Type: Journal Article
• Authors: Zhang, Lei; Cui, Haiyang; Liu, Mina; Wang, Weidong; Li, Xiujuan; Huang, He
• Is Part Of: Chemosphere. Volume 326(2023)
• Publication Date: 2023-06
• Subjects: Multi-LMWOAs -- Polycyclic aromatic hydrocarbons -- Bioremediation -- Cells characteristics -- Proteomics; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2023.138406
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic and ubiquitous pollutants that need to be solved. The low-molecular-weight organic acid (LMWOA) holds the promise to accelerate the capacity of microbes to degrade PAHs. However, the degradation mechanism(s) with multi-LMWOAs has not been understood yet, which is closer to the complex environmental biodegradation in nature. Here, we demonstrated a comprehensive cellular and proteomic response pattern by investigating the relationship between a model PAH degrading strain, B. subtili s ZL09-26, and the mixture LMWOAs (citric acid, glutaric acid, and oxalic acid). As a result, multi-LMWOAs introduced a highly enhanced phenanthrene (PHE) degradation efficiency with up to 3.1-fold improvement at 72 h, which is accompanied by the enhancement of strain growth and activity, but the releasement of membrane damages and oxidative stresses. Moreover, a detailed proteomic analysis revealed that the synergistic perturbation of various metabolic pathways jointly governed the change of cellular behaviors and improved PHE degradation in a network manner. The obtained knowledge provides a foundation for designing the artificial LMWOAs mixtures and guides the rational remediation of contaminated soils using bio-stimulation techniques. Graphical abstract: Image 1 Highlights: Revealing the responses of B. subtilis ZL09-26 in presence of multi-LMWOAs. Multi-LMWOAs improved cell growth and activity, and relieved cell damage. Multi-LMWOAs up-regulated energy and biosynthetic precursors production pathways. Multi-LMWOAs up-regulated amino acid biosynthesis. Multi-LMWOAs up-regulated ABC transporters related enzymes.