Preparation and application of novel thermo-sensitive molecularly imprinted polymer for selective extraction of omeprazole from pharmaceutical formulation and biological fluids. Issue 8 (24th May 2023)

• Record Type: Journal Article
• Authors: Derakhshan, Setareh; Panahi, Homayon Ahmad; Nikpour Nezhati, Mahshid
• Is Part Of: Separation science and technology. Volume 58:Issue 8(2023)
• Page Start: 1460
• Page End: 1472
• Publication Date: 2023-05-24
• Subjects: Molecularly imprinted polymer -- omeprazole -- N, N′-methylenebisacrylamide -- extraction -- thermo-sensitive polymer; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2023.2197552
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: Omeprazole selective molecularly imprinted polymer was synthesized by polymerization using 2-hydroxyethyl methacrylate as a monomer, N-vinylcaprolactam as a thermo-sensitive polymer, and N, N′-methylenebisacrylamide as cross-linker, respectively. This study aimed to develop a novel polymer as a nanoadsorbent for the extraction of omeprazole in biological and pharmaceutical samples. To achieve maximum extraction recovery of the drug, the Nd:YAG pulsed laser irradiation was used to convert adsorbent particles into smaller sizes using water solvent at different irradiation times. Properties of prepared thermo-sensitive molecularly imprinted polymer were characterized by different analyses. The optimal extraction conditions were thermo-sensitive molecularly imprinted polymer dosage of 0.01 g, a cross-linking agent of 0.15 g, contact time of 15 min, eluent of methanol, the temperature of 25°C, initial drug concentration of 20 mg L −1, and solution pH of 4. The sorption isotherms and selective recognition of thermo-sensitive molecularly imprinted polymer were investigated in detail. Sorption results fitted well into the linearized Langmuir equation. The selectivity experiment has shown that the sorption capacity and selectivity of thermo-sensitive molecularly imprinted polymer to omeprazole were higher than those of bicalutamide and prednisolone. A high-performance liquid chromatography-ultra-violet detector method was validated and utilized for analysis of drug extracted from biological and pharmaceutical samples. The method was linear in the range of 0.5–80 mg L −1 with a relative standard deviation value of 0.094 and a limit of detection and a limit of quantification of 0.9 and 30 µg L −1, respectively. The recovery of omeprazole drug ranged from 94.3%, 99.2%, and 98.1%, respectively, in human plasma, human urine, and pharmaceutical samples.