Degradation performance and potential protection mechanism of the anammox consortia in response to capecitabine. (June 2023)

• Record Type: Journal Article
• Authors: Wang, Xiaojing; Chen, Duxiong; Zhou, Yufei; Yu, Mingchuan; Niu, Junfeng
• Is Part Of: Chemosphere. Volume 327(2023)
• Publication Date: 2023-06
• Subjects: Anammox -- Capecitabine -- Sialic acid -- Metabolic pathway; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2023.138539
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The potential risks of anti–cancer drugs such as capecitabine have attracted considerable attention due to their continuous release. Understanding the response of removal performance and protective mechanism to the presence of emerging contaminants is crucial for the application of anammox techniques in wastewater treatment. Capecitabine affected the nitrogen removal performance slightly in the activity experiment. Due to bio–adsorption and biodegradation, up to 64–70% of the capecitabine can be removed effectively. However, 10 mg/L of capecitabine significantly decreased the removal efficiency of capecitabine and total nitrogen at repeated load of capecitabine. Metabolomic analysis revealed the metabolites 5′–deoxy–5–fluorocytidine and alpha–fluoro–beta–alanine, while metagenomic analysis confirmed the biodegradation pathway and underlying gene distribution. The potentially protective mechanisms of the system against capecitabine were the increased heterotrophic bacteria and secretion of sialic acid. Blast analysis confirmed the presence of potential genes involved in the complete biosynthesis pathway of sialic acid in anammox bacteria, some of which are also found in Nitrosomonas, Thauera, and Candidatus Promineofilum . Graphical abstract: Image 1 Highlights: Anammox performance in response to capecitabine were examined. Capecitabine can be removed due to bio-adsorption and biodegradation. The potential protective mechanism was secretion of sialic acid. Potential genes of complete sialic acid biosynthesis exist in anammox bacteria.