Atomic force microscopy study of the complexation of sterols and the glycoalkaloid α-tomatine in Langmuir-Blodgett monolayers. (May 2023)

• Record Type: Journal Article
• Authors: Nepal, Bishal; Stine, Keith J.
• Is Part Of: Chemistry and physics of lipids. Volume 252(2023)
• Publication Date: 2023-05
• Subjects: Monolayer -- Cholesterol -- Glycoalkaloid -- Atomic force microscopy -- Langmuir-Blodgett film; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipids; Periodicals; Electronic journals; 547.77
• DOI: 10.1016/j.chemphyslip.2023.105293
• Languages: English
• ISSNs: 0009-3084

Abstract

Abstract: Glycoalkaloids are secondary metabolites produced by plants that aid in their protection from pathogens and pests. They are known to form 1:1 complexes with 3β-hydroxysterols such as cholesterol causing membrane disruption. So far, the visual evidence showcasing the complexes formed between glycoalkaloids and sterols in monolayers has been mainly restricted to some earlier studies using Brewster angle microscopy which were of low resolution showing the formation of floating aggregates of these complexes. This study is aimed at using atomic force microscopy (AFM) for topographic and morphological analysis of the aggregates of these sterol-glycoalkaloid complexes. Langmuir-Blodgett (LB) transfer of mixed monolayers of the glycoalkaloid α-tomatine, sterols, and lipids in varying molar ratios onto mica followed by AFM examination was performed. The AFM method allowed visualization of the aggregation of sterol-glycoalkaloid complexes at nanometer resolution. While aggregation was observed in mixed monolayers of α-tomatine with cholesterol and in mixed monolayers with coprostanol, no sign of complexation was observed for the mixed monolayers of epicholesterol and α-tomatine, confirming their lack of interaction found in prior monolayer studies. Aggregates were observed in transferred monolayers of ternary mixtures of α-tomatine with cholesterol and the phospholipids 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) or egg sphingomyelin (egg SM). The formation of aggregates was found to be less prevalent for mixed monolayers of DMPC and cholesterol containing α-tomatine than it was for mixed monolayers containing egg SM and cholesterol with α-tomatine. The observed aggregates were generally elongated structures, of a width ranging from about 40–70 nm. Highlights: Atomic force microscopy used to image aggregates of α-tomatine/sterol complexes in Langmuir-Blodgett films on mica. Complex formation and aggregation seen for α-tomatine with cholesterol but not with epicholesterol. Bearing analysis used to estimate area fraction of aggregated complexes in selected mixtures. Evidence for stronger interaction between cholesterol and sphingomyelin than between cholesterol and phospholipid.