Cost-effective, scalable production of glucose oxidase using Casuarina equisetifolia biomass and its application in the bio-Fenton oxidation process for the removal of trace organic contaminants from wastewater. (June 2023)

• Record Type: Journal Article
• Authors: Vaidyanathan, Vinoth Kumar; Alanazi, Abdullah K.; Senthil Kumar, P.; Rajendran, Devi Sri; Chidambaram, Ashok; Venkataraman, Swethaa; Kumar, Vaithyanathan Vasanth; Rangasamy, Gayathri; Cabana, Hubert; Abo-Dief, Hala M.
• Is Part Of: Bioresource technology. Volume 377(2023)
• Publication Date: 2023-06
• Subjects: Glucose oxidase -- Trace organic compounds -- Medium optimization -- Wastewater -- bio-Fenton; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2023.128958
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Casuarina equisetifolia biomass was used for production of glucose oxidase. Pilot scale glucose oxidase production from Aspergillus niger was demonstrated. 602 U/mL of glucose oxidase (GOD) was produced after 96 h of fermentation time. 544.3 mg/L of GOD assisted H2 O2 production was optimized by RSM. GOD-driven H2 O2 was used in bio-Fenton process removed 36–89% of TrOCs. Abstract: This study focuses on using Casuarina equisetifolia biomass for pilot-scale glucose oxidase production from Aspergillus niger and its application in the removal of trace organic contaminants (TrOCs) from municipal wastewater through the bio-Fenton oxidation. The cost of glucose oxidase was 0.005 $/U, including the optimum production parameters, 10% biomass, 7% sucrose, 1% peptone, and 3% CaCO3 at 96 h with an enzyme activity of 670 U/mL. Optimized conditions for H2 O2 were 1 M glucose, 100 U/mL glucose oxidase, and 120 mins of incubation, resulting in 544.3 mg/L H2 O2 . Thus, H2 O2 produced under these conditions lead to bio-Fenton oxidation resulting in the removal of 36–92% of nine TrOCs in municipal wastewater at pH 7.0 in 360 mins. Therefore, this work establishes the cost-effective glucose oxidase-producing H2 O2 as an attractive bioremediating agent to enhance the removal of TrOCs in wastewater at neutral pH.