Fecal microbiota transplantation reduces mouse mortality from Listeria monocytogenes infection. (May 2023)

• Record Type: Journal Article
• Authors: Guo, Liang; Yin, Xianhong; Liu, Qing
• Is Part Of: Microbial pathogenesis. Volume 178(2023)
• Publication Date: 2023-05
• Subjects: Listeria monocytogenes -- Infection -- Gut microbiota -- FMT -- Reduce -- Mortality; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2023.106036
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Listeria monocytogenes (Lm) is a food bacterium with strong pathogenicity which causes infections via the gastrointestinal tract. Mechanisms by which gut microbiota (GM) resist microbial infections have received little attention. Eight-week-old mice were orally inoculated with wild-type Lm EGD-e and fecal microbiota transplantation (FMT) employed. GM richness and diversity of infected mice changed rapidly within 24h. Firmicutes class decreased and Bacteroidetes, Tenericutes and Ruminococcaceae increased significantly. Coprococcus, Blautia and Eubacterium also increased on the 3rd day post-infection. Moreover, GM transplanted from healthy mice reduced mortality of infected mice by approximately 32%. FMT treatment decreased production of TNFα, IFN-γ, IL-1β and IL-6 relative to PBS treatment. In summary, FMT has potential as a treatment against Lm infection and may be used for bacterial resistance management. Further work is required to elucidate the key GM effector molecules. Highlights: Inoculated with EGD-e altered the gut microbiota diversity significantly in mice, and the alteration was different in the different stages of Lm infection. The gut microbiota of healthy mice could reduce the mortality of infected mice. The gut microbiota of healthy mice could reduce the expression of pro-inflammatory cytokines of Lm-infected mice.