New insights into multi-strategies of sludge granulation in up-flow anaerobic sludge blanket reactors from community succession and interaction. (June 2023)

• Record Type: Journal Article
• Authors: Xiao, Yeyuan; Wang, Xucai; Wang, Peiling; Zhou, Zhongbo; Wang, Hui; Teng, Tao; Li, Yiwei; Yang, Lei
• Is Part Of: Bioresource technology. Volume 377(2023)
• Publication Date: 2023-06
• Subjects: Quorum sensing -- Acyl-homoserine lactone -- Phylogenetic molecular ecological network -- Chemotaxis attraction -- Second messengers; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2023.128935
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Four stages in sludge granulation: selection, seeding, expansion, and maturation. Low-abundance (<1%) taxa largely governed community succession in granulation. Chemotaxis and flagella assembly were the primary driving force of granulation. cGMP and cAMP signaling pathways regulated the initial assembly of inner cores. Quorum sensing mediated the rapid expansion of granules prior to maturation. Abstract: This study aimed to elucidate the multiple strategies employed by anaerobes during granulation in a laboratory upflow anaerobic sludge blanket reactor, based on microbial succession and interactions. The anaerobic granulation process featured staged dominance of microbial genera, corresponding well with the environmental traits. Across the stages (selection, seeding, expansion, and maturation), chemotaxis attraction of nitrogen and/or carbon sources and flagellar motion were the primary strategy of microbial assembly. The second messengers – cyclic adenosine and guanosine monophosphates – partially regulated the agglomeration of filamentous Euryachaeota and Chloroflexi as the inner cores, while quorum sensing mediated the expansion of granules prior to maturation. Antagonism or competition governed the interactions within the phylogenetic molecular ecological network during sludge granulation, which were largely driven by the low-abundance (<1%) taxa. These new insights suggest that better engineering solutions to enhance chemotaxis attraction and species selection could achieve more efficient anaerobic granular sludge processes.