Fine-tuning the sequential drug release of nano-formulated mutual prodrugs dictates the combination effects. Issue 14 (17th March 2023)

• Record Type: Journal Article
• Authors: Zhong, Haiping; Li, Xingwei; Yu, Na; Zhang, Xi; Mu, Jingqing; Liu, Tao; Yuan, Bo; Yuan, Xiaoyong; Guo, Shutao
• Is Part Of: Chemical science. Volume 14:Issue 14(2023)
• Page Start: 3789
• Page End: 3799
• Publication Date: 2023-03-17
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d3sc00550j
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : Nanoformulated mutual prodrugs able to release two drugs either in order or simultaneously which significantly affected the combination effects consistently in vitro and in vivo, and links the in vitro – in vivo optimization of therapeutic effects. Abstract : The maintenance of robust ratiometric loading of dual therapeutic agents and fine-tuning release kinetics for consistent in vitro and in vivo optimization of combination effects is vital for discovering new anticancer drug combinations and remains challenging. Smart nanomedicine strategies have been investigated for this purpose, but most of the reported strategies focus either on ratiometric delivery or on unimodal sequential release of the two different agents, which hampers effective optimization of combination effects. Herein we report a sequential drug release system based on nanoformulated mutual prodrugs constructed by the formation of ketal linkages with different acid sensitivities, thus enabling the acid-triggered release of two anticancer drugs, paclitaxel and gemcitabine, in various sequences. We found that in several cell lines, the sequence of drug release substantially affected the combination effects; specifically, in A549 cells, time-staggered release profiles showed enhanced synergistic effects relative to those of a simultaneous release profile. Moreover, in vivo assessment of the antitumor efficacy of the nanoformulations in A549 xenograft models indicated that the best therapeutic effects were obtained with time-staggered release profiles, which was consistent with the in vitro results. Our strategy for precisely controlled sequential drug release can be expected to facilitate the screening of optimal drug combinations and maximize combination effects both in vitro and in vivo .