Effect of BsaA I genotyped intronic SNP of leptin gene on production and reproduction traits in Indian dairy cattle. Issue 2 (3rd April 2023)

• Record Type: Journal Article
• Authors: Yadav, Tejwanti; Magotra, Ankit; Bangar, Yogesh. C.; Kumar, Ramesh; Yadav, A. S.; Garg, Asha Rani; Bahurupi, Pooja; Kumar, Pankaj
• Is Part Of: Animal biotechnology. Volume 34:Issue 2(2023)
• Page Start: 261
• Page End: 267
• Publication Date: 2023-04-03
• Subjects: Leptin -- Sahiwal -- genotype -- polymorphism; Animal biotechnology -- Periodicals; Animals, Domestic -- genetics -- Periodicals; Animals, Genetically Modified -- Periodicals; 660.6
• DOI: 10.1080/10495398.2021.1955701
• Languages: English
• ISSNs: 1049-5398

Abstract

Abstract: In the present study, candidate single nucleotide polymorphism (SNP) g.92450765 G > A of leptin gene was explored for Bos indicus cattle with an aim to explore its possible effect on production and reproduction traits. The genotypic and allelic frequencies of BsaA 1 genotyped SNP g.92450765 G > A indicated the predominance of AG (0.65) genotyped Sahiwal cows in our studied population. The least-squares analysis showed a significant association ( p < 0.05) of identified genetic variants with total milk yield (TMY) and 305 days milk yield (MY) ( p < 0.05). The GG genotyped cows were found to be associated with higher milk yields. However, for reproduction traits under study AA genotype was found to be more favorable with respect to service period and calving interval ( p < 0.05). Computational analysis was also performed to predict changes if any in the transcription factor binding sites (TFBS) caused due to the identified SNP in the intronic region of the leptin gene. TFBS analysis predicted that the targeted SNP at g.92450765G > A may lead to the disappearance of TFBS such as Hypermethylated in cancer 2 protein (HIC2), Max-binding protein MNT (MNT), Cyclic AMP-dependent transcription factor −3 (ATF3), Myc-associated factor X (MAX) and Microphthalmia-associated transcription factor (MITF). It may lead to changes in transcriptional regulation.